General Information

  • ID:  hor004967
  • Uniprot ID:  P83659
  • Protein name:  Cyphokinin
  • Gene name:  NA
  • Organism:  Cyphononyx dorsalis (Spider wasp) (Cyphononyx fulvognathus)
  • Family:  Bradykinin-related peptide family
  • Source:  animal
  • Expression:  Expressed by the venom gland.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Cyphononyx (genus), Pepsinae (subfamily), Pompilidae (family), Pompiloidea (superfamily), Aculeata (infraorder), Apocrita (suborder), Hymenoptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003674 molecular_function; GO:0005179 hormone activity; GO:0090729 toxin activity
  • GO BP:  GO:0006952 defense response; GO:0007165 signal transduction; GO:0035821 modulation of process of another organism; GO:0045776 negative regulation of blood pressure; GO:0045987 positive regulation of smooth muscle contraction
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

  • Sequence:  DTRPPGFTPFR
  • Length:  11
  • Propeptide:  DTRPPGFTPFR
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  inhibits angiotensin-converting enzyme (ACE) with a Ki of 2.2 ¦ÌM and targets B2 bradykinin receptor (BDKRB2). Also provokes contraction of smooth muscle preparation (ileum). In vivo, induces hyperalgesic effects in living rats after intraplantar injection
  • Mechanism:  Cyphokinin is hydrolyzed by ACE by only 27.3% (compared to bradykinin).
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: 352.5+/-64.7nM
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004967_AF2.pdbhor004967_ESM.pdb

Physical Information

Mass: 146897 Formula: C59H87N17O16
Absent amino acids: ACEHIKLMNQSVWY Common amino acids: P
pI: 10.4 Basic residues: 2
Polar residues: 3 Hydrophobic residues: 2
Hydrophobicity: -122.73 Boman Index: -3680
Half-Life: 1.1 hour Half-Life Yeast: 3 min
Half-Life E.Coli: >10 hour Aliphatic Index 0
Instability Index: 4795.45 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  19716363
  • Title:  Bradykinin-related peptides in the venom of the solitary wasp Cyphononyx fulvognathus.